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| Mendoza,Héctor; de la Jara,Adelina; Freijanes,Karen; Carmona,Laura; Ramos,Ana Alexandra; de Sousa Duarte,Vanessa; Serafim Varela,João Carlos. |
| In the present work the characterization of different strains of Dunaliella salina from established cell culture collections and various isolates from solar saltworks located in the Canary Island, as well as one mutant, was carried out in order to assess the existence of intraspecific differences and to determine the potential productivity of each one. Morphological characteristics such as cellular size and cellular complexity and parameters linked to cellular physiology, such as pigment content or cellular growth rate, were determined by means of traditional techniques as well as flow cytometry. Results showed a high morphological and physiological intraspecific variability among the studied strains. Results suggest that the application of the lipidic dye... |
| Tipo: Journal article |
Palavras-chave: Carotene; Carotenogenesis; Dunaliella salina; Flow cytometry; Nile red. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400005 |
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| CIFUENTES,ANA S; GONZÁLEZ,MARIELA A; VARGAS,SILVIA; HOENEISEN,MARITZA; GONZÁLEZ,NELSON. |
| The microalga Haematococcus pluvialis Flotow is one of the natural sources of astaxanthin, a pigment widely used in salmon feed. This study was made to discover optimal conditions for biomass and astaxanthin production in H. pluvialis from Steptoe, Nevada (USA), cultured in batch mode. Growth was carried out under autotrophic (with NaNO3, NH4Cl and urea) and mixotrophic conditions (with 4, 8, 12 mM sodium acetate) under two photon flux densities (PFD) (35 and 85 µmol m-2 s-1). The carotenogenesis was induced by 1) addition of NaCl (0.2 and 0.8 %), 2) N-deprivation and 3) high PFD (150 µmol m-2 s-1). Total carotenoids were estimated by spectrophotometry and total astaxanthin by HPLC. Ammonium chloride was the best N-source for growth (k=0.7 div day-1,... |
| Tipo: Journal article |
Palavras-chave: Astaxanthin; Carotenogenesis; Growth; Haematococcus pluvialis. |
| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300006 |
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